王亮生 研究组花卉生理与遗传育种研究组

研究组长 王亮生 研究员
 
1985-1987年湘潭大学化工系教学秘书;1987-1996年湖南省教育委员会高教处、科技处副主任科员、主任科员;1996-1997年湖南农业大学园艺系任教;1996-2001年日本鹿儿岛大学农学部观赏园艺学研究室留学,获农学博士学位;2001-2004年在鹿儿岛中国经济交流协同组合工作,讲师;2004年至今中科院植物所北京植物园工作。中国花卉协会牡丹芍药分会常务理事,中国园艺学会理事。
 
联系方式: E-mail: wanglsh%40ibcas.ac.cn;; 电话: 010-62836654
 
主要研究方向:
 
1、 牡丹芍药种质资源收集保存和系统评价经过8年努力,从国内外引种牡丹800余个、芍药240余个品种,包括牡丹全部6个品种群的资源,成活率达90%以上,建成了拥有资源数量最多的牡丹和芍药种质资源圃。建立了牡丹56个农艺性状数据库和牡丹花芽cDNA文库,获得了2241个EST序列并对其进行了功能注释。
 
2、 花果色泽表型分析及其形成机制研究花果色泽评价与形成机制研究,是园艺植物品质管理和种质创新的重要科学基础之一。对我国传统名花牡丹、荷花、睡莲等和蓝莓、蓝靛果等20余种花卉、果树植物的色泽表型、色素组成进行了详细调查,为上述特色植物品质育种奠定了科学基础。
 
3、 牡丹和蓝莓的全株利用牡丹和蓝莓全身是宝。牡丹籽可榨油,牡丹油中不饱和脂肪酸90%以上,其中α-亚麻酸高达40%;花粉可用于前列腺炎治疗药物开发;丹皮是著名的中药原料。目前正在开展牡丹根皮、花粉、花瓣、种籽功能成分分析评价并探索其加工新工艺。蓝莓果中富含防癌、抗衰老、提高视力、改善记忆等优质天然花青素成分,叶中富含对丙型肝炎有显著治疗效果的高聚原花青素。目前主要对中国野生种及150余个引进品种的功能成分进行分析,探索其有效防癌和抗衰老的最佳配方和分子机理。
 
主要科研项目:
 
[1]“牡丹分子育种与品种创制”,“十二五”农村领域国家科技计划课题(863计划)(2011.01-2015.12),研究任务负责人。 
[2]“睡莲类黄酮糖基转移酶基因克隆及功能验证”,国家自然科学基金面上项目(2015.01-2018.12),项目主持人。 
[3]“利用细菌宿主生产来自浆果的生物活性酚”,欧盟第七框架项目(2013.11-2016.11),中方负责人。 
[4]“武陵山区生物多样性综合科学考察:武陵山西部植物考察”,科技部科技基础性工作专项课题(2014.05-2019.04),课题主持人。 
[5]“华北地区本土植物清查与保护”,中国科学院科技服务网络计划(STS计划)(中国植物园联盟建设专项研发与联合攻关服务)(2013.06-2016.05),子课题主持人。
研究组工作人员:
 
    任红旭  副研究员  
    徐文忠  副研究员
    李珊珊  助理研究员
    姜联合  高级工程师
    肖红强  高级技工
发表论文:
 
Wu J, Wang XC, Liu Y, Du H, Shu QY, Su S, Wang LJ, Li SS*, Wang LS*. Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation. Scientific Reports, 2016, 6:19245
 
Wu Q, Wu J, Li SS*, Zhang HJ, Feng CY, Yin DD, Wu RY, Wang LS*. Transcriptome sequencing and metabolite analysis for revealing the blue flower formation in waterlily. Bmc Genomics, 2016, 17
 
Su S, Wang LJ, Feng CY, Liu Y, Li CH, Du H, Tang ZQ, Xu YJ, Wang LS*. Fingerprints of anthocyanins and flavonols of Vaccinium uliginosum berries from different geographical origins in the Greater Khingan Mountains and their antioxidant capacities. Food Control, 2016, 64: 218-225
 
Feng CY, Li SS*, Yin DD, Zhang HJ, Tian DK, Wu Q, Wang LJ, Su S, Wang LS*. Rapid determination of flavonoids in plumules of sacred lotus cultivars and assessment of their antioxidant activities. Ind Crop Prod, 2016, 87: 96-104
 
Feng CY, Su S, Wang LJ, Wu J, Tang ZQ, Xu YJ, Shu QY, Wang LS*. Antioxidant capacities and anthocyanin characteristics of the black-red wild berries obtained in Northeast China. Food Chemistry, 2016, 204: 150-158
 
Wang LJ, Wu J, Wang HX*, Li SS, Zheng XC, Du H, Xu YJ, Wang LS*. 2015. Composition of phenolic compounds and antioxidant activity in the leaves of blueberry cultivars. Journal of Functional Foods, 16: 295-304
 
Yin DD, Yuan RY, Wu Q, Li SS, Shao S, Xu YJ, Hao XH, Wang LS*. 2015. Assessment of flavonoids and volatile compounds in tea infusions of water lily flowers and their antioxidant activities. Food Chemistry, 187: 20-28
 
Li SS, Wang LS*, Shu QY, Wu J, Chen LG, Shao S, Yin DD. 2015. Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development. BMC Genomics, DOI 10.1186/s12864-015-1429-0
 
Du H, Li SS, Wu Q, Ji KX, Wu J, Liu Y, Wang LS*. 2015. Analysis of active compounds and antioxidant activity assessment of six popular Chinese Juhua teas. Natural Product Communications, 10(3): 495-498
 
Li SS, Yuan RY, Chen LG, Wang LS*, Hao XH*, Wang LJ, Zheng XC, Du H. 2015. Systematic qualitative and quantitative assessment of fatty acids in the seeds of 60 tree peony (Paeonia section Moutan DC.) cultivars by GC-MS. Food Chemistry, 173: 133-140
 
Li SS, Wu J, Chen LG, Xu YJ, Wang LJ, Zhang HJ, Zheng XC, Wang LS*. 2014. Biogenesis of C-glycosyl flavones and profiling of flavonoid glycosides in lotus (Nelumbo nucifera). PLoS ONE, 9(10): e108860
 
Wang LJ, Su S, Wu J, Du H, Li SS, Huo JW, Zhang Y, Wang LS*. 2014. Variation of anthocyanins and flavonols in Vaccinium uliginosum berry in Lesser Khingan Mountains and its antioxidant activity. Food Chemistry, 160: 357-364
 
Yuan RY, Li SS, Zheng XC, Wu Q, Zhang HJ, Wang LS*. 2014. Determination of volatiles in water lily flowers using gas chromatography – mass spectrometry. Analytical Letters, 47(9): 1541-1551
 
Lü PT, Zhang CQ, Liu JT, Liu XW, Jiang GM, Jiang XQ, Ali KM, Wang LS, Hong B, Gao JP*. 2014. RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose (Rosa hybrida) petal senescence. The Plant Journal, 78(4): 578-590
 
Du H, Wu J, Li H, Zhong PX, Xu YJ*, Li CH, Ji KX, Wang LS*. 2013. Polyphenols and triterpenes from Chaenomeles fruits: Chemical analysis and antioxidant activities assessment. Food Chemistry, 141(4): 4260-4268
 
Gao ZM*, Wu J, Liu ZA, Wang LS, Ren HX, Shu QY*. 2013. Rapid microsatellite development for tree peony and its implications. BMC Genomics, 14(1): 886-897
 
Liu NN, Sun GF, Xu YJ, Luo ZF, Lin QW, Li XD*, Zhang JZ*, Wang LS*. 2013. Anthocyanins of the genus of Hosta and their impacts on tepal colors. Scientia Horticulturae, 150: 172-180
 
Zhu ML, Zheng XC, Shu QY*, Li H, Zhong PX, Zhang HJ, Xu YJ, Wang LJ, Wang LS*. 2012. Relationship between the composition of flavonoids and flower colors variation in Tropical Water Lily (Nymphaea) cultivars. PLoS ONE, 7(4): e34335
 
Zhang J, Wang LS*, Gao JM*, Xu YJ, Li LF, Li CH. 2012. Rapid separation and identification of anthocyanins from flowers of Viola yedoensis and V. prionantha by high performance liquid chromatography – photodiode array detection – electrospray ionization mass spectrometry. Phytochemical Analysis, 23(1): 16-22
 
Zhang JJ, Shu QY*, Liu ZA, Ren HX, Wang LS*, De Keyser E. 2012. Two EST-derived marker systems for cultivar identification in tree peony. Plant Cell Reports, 31(2): 299-310
 
Shu QY*, Wang LS, Wu J, Du H, Liu ZA, Ren HX*, Zhang JJ. 2012. Analysis of the formation of flower shapes in wild species and cultivars of tree peony using the MADS-box subfamily gene. Gene, 493(1): 113-123
 
He QL, Shen Y, Wang MX, Huang MR*, Yang RZ, Zhu SJ, Wang LS*, Xu YJ, Wu RL*. 2011. Natural variation in petal color in Lycoris longituba revealed by anthocyanin components. PLoS ONE, 6(8): e22098
 
Zheng J, Ding CX, Wang LS, Li GL, Shi JY, Li H, Wang HL, Suo YR*.  2011. Anthocyanins composition and antioxidant activity of wild Lycium ruthenicum Murr. from Qinghai-Tibet plateau. Food Chemistry, 126: 859-865
 
Zheng J, Li H, Ding CX, Suo YR*, Wang LS, Wang HL. 2011. Anthocyanins composition and antioxidant activity of two major wild Nitraria tangutorun Bobr. variations from Qinghai-Tibet plateau. Food Research International, 44: 2041-2046 
 
 
著作专著:
 
[1]王亮生,程金水,马江生,2010。第3章 花色与彩斑的遗传。程金水、刘青林主编:《园林植物遗传育种学》(第2版)。北京:中国林业出版社。(中文)
 
[2]Wang LS, Liu ZA, Shu QY, Tian DK, Han XY, Zhang JJ, Zhang Z, Li CH (translated). 2005. Foreword, Preface, Introduction, Chapter 1, Chapter 3, Chapter 5. Chinese Tree Peony (Xibei, Xinan, Jiangnan Volume). Jia-Jue Li (wrote), Chun-Neng Wang et al. (translated). China Forestry Publishing House. pp.1-15, 26-35, 43-52
 
[3]刘政安,王亮生,张丽萍,舒庆艳,韩小燕,2005。丹皮产业化发展中存在的问题与对策.《中医药发展与现代科学技术》,上册。成都:四川科学技术出版社。P.312-316(中文)
 
[4]王亮生,1999。牡丹的花色、花色素与遗传育种。李嘉珏主编:《中国牡丹与芍药》。北京:中国林业出版社。P.103-108(中文)
 
 
授权专利:
 
[1]舒庆艳,张晶晶,王亮生. 获取与牡丹花型性状相关的分子标记的方法与专用引物:中国,200910077930.6
 
[2]王亮生,张晶晶,舒庆艳. 牡丹的一种DNA指纹图谱及其获取方法与专用引物:中国,200910077931.0
 
[3]Wang Liangsheng, Du Hui, Shu Qingyan. Novel protein and gene related to flavonoid O-methyltransferase (FOMT) and their uses therefore. PCT/CN2013/074973.(国际专利申请)